From: owner-ammf-digest@smoe.org (alt.music.moxy-fruvous digest) To: ammf-digest@smoe.org Subject: alt.music.moxy-fruvous digest V14 #4457 Reply-To: ammf@fruvous.com Sender: owner-ammf-digest@smoe.org Errors-To: owner-ammf-digest@smoe.org Precedence: bulk alt.music.moxy-fruvous digest Saturday, June 27 2020 Volume 14 : Number 4457 Today's Subjects: ----------------- Triple Impact EDC Tool? ["Survival Situation" Subject: Triple Impact EDC Tool? Triple Impact EDC Tool? http://farmthermo.guru/W5ZNn0g0vuFW_8pRRn9sCs0RPQg2lkrPorXatd1dDR6sP_MA http://farmthermo.guru/qaW_5UIbo_DE4ig_CdVSm6RDFxM7z2InEcxmDNnYdNIjhR9H Research on emotion has increased significantly over the past two decades with many fields contributing including psychology, neuroscience, affective neuroscience, endocrinology, medicine, history, sociology of emotions, and computer science. The numerous theories that attempt to explain the origin, neurobiology, experience, and function of emotions have only fostered more intense research on this topic. Current areas of research in the concept of emotion include the development of materials that stimulate and elicit emotion. In addition, PET scans and fMRI scans help study the affective picture processes in the brain. From a purely mechanistic perspective, "Emotions can be defined as a positive or negative experience that is associated with a particular pattern of physiological activity." Emotions produce different physiological, behavioral and cognitive changes. The original role of emotions was to motivate adaptive behaviors that in the past would have contributed to the passing on of genes through survival, reproduction, and kin selection. In some theories, cognition is an important aspect of emotion. For those who act primarily on emotions, they may assume that they are not thinking, but mental processes involving cognition are still essential, particularly in the interpretation of events. For example, the realization of our believing that we are in a dangerous situation and the subsequent arousal of our body's nervous system (rapid heartbeat and breathing, sweating, muscle tension) is integral to the experience of our feeling afraid. Other theories, however, claim that emotion is separate from and can precede cognition. Consciously experiencing an emotion is exhibiting a mental representation of that emotion from a past or hypothetical experience, which is linked back to a content state of pleasure or displeasure. The content states are established by verbal explanations of experiences, describing an internal state. Emotions are complex. According to some theories, they are states of feeling that result in physical and psychological changes that influence our behavior. The physiology of emotion is closely linked to arousal of the nervous system with various states and strengths of arousal relating, apparently, to particular emotions. Emotion is also linked to behavioral tendency. Extroverted people are more likely to be social and express their emotions, while introverted people are more likely to be more socially withdrawn and conceal their emotions. Emotion is often the driving force behind motivation, positive or negative. According to other theories, emotions are not causal forces but simply syndromes of components, which might include motivation, feeling, behavior, and physiological changes, but no one of these components is the emotion. Nor is the emotion an entity that causes these components. Emotions involve different components, such as subjective experience, cognitive processes, expressive behavior, psychophysiological changes, and instrumental behavior. At one time, academics attempted to identify the emotion with one of the components: William James with a subjective experience, behaviorists with instrumental behavior, psychophysiologists with physiological changes, and so on. More recently, emotion is said to consist of all the components. The different components of emotion are categorized somewhat differently depending on the academic discipline. In psychology and philosophy, emotion typically includes a subjective, conscious experience characterized primarily by psychophysiological expressions, biological reactions, and mental states. A similar multicomponential description of emotion is found in sociology. For example, Peggy Thoits described emotions as involving physiological components, cultural or emotional labels (anger, surprise, etc.), expressive body actions, and the appraisal of situations and contexts. ------------------------------ Date: Sat, 27 Jun 2020 04:41:52 -0400 From: "Survival Situation" Subject: Triple Impact EDC Tool? Triple Impact EDC Tool? http://farmthermo.guru/xuvYW6sHTzuQMhMP1oPBMEQ4lZxt-_kKUX4qQl6L4F3hvfYF http://farmthermo.guru/nPDaE6Lu_rO8axN8F_Ed6ObuRjQwQpRGJaSHlJnGMxx1A4jr Research on emotion has increased significantly over the past two decades with many fields contributing including psychology, neuroscience, affective neuroscience, endocrinology, medicine, history, sociology of emotions, and computer science. The numerous theories that attempt to explain the origin, neurobiology, experience, and function of emotions have only fostered more intense research on this topic. Current areas of research in the concept of emotion include the development of materials that stimulate and elicit emotion. In addition, PET scans and fMRI scans help study the affective picture processes in the brain. From a purely mechanistic perspective, "Emotions can be defined as a positive or negative experience that is associated with a particular pattern of physiological activity." Emotions produce different physiological, behavioral and cognitive changes. The original role of emotions was to motivate adaptive behaviors that in the past would have contributed to the passing on of genes through survival, reproduction, and kin selection. In some theories, cognition is an important aspect of emotion. For those who act primarily on emotions, they may assume that they are not thinking, but mental processes involving cognition are still essential, particularly in the interpretation of events. For example, the realization of our believing that we are in a dangerous situation and the subsequent arousal of our body's nervous system (rapid heartbeat and breathing, sweating, muscle tension) is integral to the experience of our feeling afraid. Other theories, however, claim that emotion is separate from and can precede cognition. Consciously experiencing an emotion is exhibiting a mental representation of that emotion from a past or hypothetical experience, which is linked back to a content state of pleasure or displeasure. The content states are established by verbal explanations of experiences, describing an internal state. Emotions are complex. According to some theories, they are states of feeling that result in physical and psychological changes that influence our behavior. The physiology of emotion is closely linked to arousal of the nervous system with various states and strengths of arousal relating, apparently, to particular emotions. Emotion is also linked to behavioral tendency. Extroverted people are more likely to be social and express their emotions, while introverted people are more likely to be more socially withdrawn and conceal their emotions. Emotion is often the driving force behind motivation, positive or negative. According to other theories, emotions are not causal forces but simply syndromes of components, which might include motivation, feeling, behavior, and physiological changes, but no one of these components is the emotion. Nor is the emotion an entity that causes these components. Emotions involve different components, such as subjective experience, cognitive processes, expressive behavior, psychophysiological changes, and instrumental behavior. At one time, academics attempted to identify the emotion with one of the components: William James with a subjective experience, behaviorists with instrumental behavior, psychophysiologists with physiological changes, and so on. More recently, emotion is said to consist of all the components. The different components of emotion are categorized somewhat differently depending on the academic discipline. In psychology and philosophy, emotion typically includes a subjective, conscious experience characterized primarily by psychophysiological expressions, biological reactions, and mental states. A similar multicomponential description of emotion is found in sociology. For example, Peggy Thoits described emotions as involving physiological components, cultural or emotional labels (anger, surprise, etc.), expressive body actions, and the appraisal of situations and contexts. ------------------------------ Date: Sat, 27 Jun 2020 03:47:31 -0400 From: "Adult Dating" Subject: Beautiful Local Milfs Waiting for You Beautiful Local Milfs Waiting for You http://biomutual.bid/lzzaBz-uu0eXUVr8KKhJ0prbqO78U_Q5KH0sw8VfjWjZEjNT http://biomutual.bid/HH0sj0Ixu1flt_TBsdGxXAHvNcHJxI1GuCWiR_1Bnj_N1h9c Most doors are hinged along one side to allow the door to pivot away from the doorway in one direction, but not the other. The axis of rotation is usually vertical. In some cases, such as hinged garage doors, the axis may be horizontal, above the door opening. Doors can be hinged so that the axis of rotation is not in the plane of the door to reduce the space required on the side to which the door opens. This requires a mechanism so that the axis of rotation is on the side other than that in which the door opens. This is sometimes the case in trains or airplanes, such as for the door to the toilet, which opens inward. A swing door has special single-action hinges that allow it to open either outwards or inwards, and is usually sprung to keep it closed. French doors are derived from an original French design called the casement door. It is a door with lites where all or some panels would be in a casement door. A French door traditionally has a moulded panel at the bottom of the door. It is called a French window when used in a pair as double-leaved doors with large glass panels in each door leaf, and in which the doors may swing out (typically) as well as in. A Mead door, developed by S Mead of Leicester, swings both ways. It is susceptible to forced entry due to its design. A Dutch door or stable door consists of two halves. The top half operates independently from the bottom half. A variant exists in which opening the top part separately is possible, but because the lower part has a lip on the inside, closing the top part, while leaving the lower part open, is not. A garden door resembles a French window (with lites), but is more secure because only one door is operable. The hinge of the operating door is next to the adjacent fixed door and the latch is located at the wall opening jamb rather than between the two doors or with the use of an espagnolette bolt. Sliding doors It is often useful to have doors which slide along tracks, often for space or aesthetic considerations. A bypass door is a door unit that has two or more sections. The doors can slide in either direction along one axis on parallel overhead tracks, sliding past each other. They are most commonly used in closets, in order to access one side of the closet at a time. The doors in a bypass unit will overlap slightly when viewed from the front, in order not to have a visible gap between them. Doors which slide inside a wall cavity are called pocket doors. This type of door is used in tight spaces where privacy is also required. The door slab is mounted to roller and a track at the top of the door and slides inside a wall. Sliding glass doors are common in many houses, particularly as an entrance to the backyard. Such doors are also popular for use for the entrances to commercial structures, although they are not counted as fire exit doors. The door that moves is called the "active leaf", while the door that remains fixed ------------------------------ Date: Sat, 27 Jun 2020 05:19:40 -0400 From: "UV Protection Shirt" Subject: Mystery items included each month! Mystery items included each month! http://biomutual.bid/RP9KkdkSIH9cqb1X93S7OvsJoEovkHV-mWNIDvh9cjHqb0uB http://biomutual.bid/ihSAcEnwgVyGpQ92ToUpJvw6UUBjh-jzCyG7974G-ffYwBcN Ekman's most influential work revolved around the finding that certain emotions appeared to be universally recognized, even in cultures that were preliterate and could not have learned associations for facial expressions through media. Another classic study found that when participants contorted their facial muscles into distinct facial expressions (for example, disgust), they reported subjective and physiological experiences that matched the distinct facial expressions. Ekman's facial-expression research examined six basic emotions: anger, disgust, fear, happiness, sadness and surprise. Later in his career, Ekman theorized that other universal emotions may exist beyond these six. In light of this, recent cross-cultural studies led by Daniel Cordaro and Dacher Keltner, both former students of Ekman, extended the list of universal emotions. In addition to the original six, these studies provided evidence for amusement, awe, contentment, desire, embarrassment, pain, relief, and sympathy in both facial and vocal expressions. They also found evidence for boredom, confusion, interest, pride, and shame facial expressions, as well as contempt, interest, relief, and triumph vocal expressions. Robert Plutchik agreed with Ekman's biologically driven perspective but developed the "wheel of emotions", suggesting eight primary emotions grouped on a positive or negative basis: joy versus sadness; anger versus fear; trust versus disgust; and surprise versus anticipation. Some basic emotions can be modified to form complex emotions. The complex emotions could arise from cultural conditioning or association combined with the basic emotions. Alternatively, similar to the way primary colors combine, primary emotions could blend to form the full spectrum of human emotional experience. For example, interpersonal anger and disgust could blend to form contempt. Relationships exist between basic emotions, resulting in positive or negative influences. Multi-dimensional analysis Sorting emotions into unpleasant-pleasant and activated-calm. Two dimensions of emotions. Made accessible for practical use. Two dimensions of emotion Psychologists have used methods such as factor analysis to attempt to map emotion-related responses onto a more limited number of dimensions. Such methods attempt to boil emotions down to underlying dimensions that capture the similarities and differences between experiences. Often, the first two dimensions uncovered by factor analysis are valence (how negative or positive the experience feels) and arousal (how energized or enervated the experience feels). These two dimensions can be depicted on a 2D coordinate map. This two-dimensional map has been theorized to capture one important component of emotion called core affect. Core affect is not theorized to be the only component to emotion, but to give the emotion its hedonic and felt energy. ------------------------------ Date: Sat, 27 Jun 2020 03:21:08 -0400 From: "Adult Dating" Subject: Beautiful Local Ladies Waiting for You Beautiful Local Ladies Waiting for You http://farmthermo.guru/-L1uwFjUK9DBC6C96wPxV0oZZfU9qdZKnL-m_DXc_pZEdQo http://farmthermo.guru/g5F_8EFecqPJSfDCR2eTJma277hd2mVyJx7QNE5bAUmL9J73 vest in their social life" are "so much a part of all classificatory disputes about art." According to Novitz, classificatory disputes are more often disputes about societal values and where society is trying to go than they are about theory proper. For example, when the Daily Mail criticized Hirst's and Emin's work by arguing "For 1,000 years art has been one of our great civilising forces. Today, pickled sheep and soiled beds threaten to make barbarians of us all" they are not advancing a definition or theory about art, but questioning the value of Hirst's and Emin's work. In 1998, Arthur Danto, suggested a thought experiment showing that "the status of an artifact as work of art results from the ideas a culture applies to it, rather than its inherent physical or perceptible qualities. Cultural interpretation (an art theory of some kind) is therefore constitutive of an object's arthood." Anti-art is a label for art that intentionally challenges the established parameters and values of art; it is term associated with Dadaism and attributed to Marcel Duchamp just before World War I, when he was making art from found objects. One of these, Fountain (1917), an ordinary urinal, has achieved considerable prominence and influence on art. Anti-art is a feature of work by Situationist International, the lo-fi Mail art movement, and the Young British Artists, though it is a form still rejected by the Stuckists, who describe themselves as anti-anti-art. Architecture is often included as one of the visual arts; however, like the decorative arts, or advertising, it involves the creation of objects where the practical considerations of use are essential in a way that they usually are not in a painting, for example. Value judgment Aboriginal hollow log tombs. National Gallery, Canberra, Australia Somewhat in relation to the above, the word art is also used to apply judgments of value, as in such expressions as "that meal was a work of art" (the cook is an artist), or "the art of deception", (the highly attained level of skill of the deceiver is praised). It is this use of the word as a measure of high quality and high value that gives the term its flavor of subjectivity. Making judgments of value requires a basis for criticism. At the simplest level, a way to determine whether the impact of the object on the senses meets the criteria to be considered art is whether it is perceived to be attractive or repulsive. Though perception is always colored by experience, and is necessarily subjective, it is commonly understood that what is not somehow aesthetically satisfying cannot be art. However, "good" art is not always or even regularly aesthetically appealing to a majority of viewers. In other words, an artist's prime motivation need not be the pursuit of the aesthetic. Also, art often depicts terrible images made for social, moral, or thought-provoking reasons. For example, Francisco Goya's painting depicting the Spanish shootings of 3 May 1808 is a graphic depiction of a firing squad executing several pleading civilians. Yet at the same time, the horrific imagery demonstrates Goya's keen artistic ability in composition and execution and produces fitting social and political outrage. Thus, the debate continues as to what mode of aesthetic satisfaction, if any, is required to define 'art'. The assumption of new values or the rebellion against accepted notions of what is aesthetically superior need not occur concurrently with a complete abandonment of the pursuit of what is aesthetically appealing. Indeed, the reverse is often true, that the revision of what is popularly conceived of as being aesthetically appealing allows for a re-invigoration of aesthetic sensibility, and a new appreciation for the standards of art itself. Countless schools have proposed their own ways to define quality, yet they all seem to agree in at least one point: once their aesthetic choices are accepted, the value of the work of art is determined by its capacity to transcend the limits of its chosen medium to strike some universal chord by the rarity of the skill of the artist or in its accurate reflection in what is termed the zeitgeist. Art is often intended to appeal to and connect with human emotion. It can arouse aesthetic or moral feelings, and can be understood as a way of communicating these feelings. Artists express something so that their audience is aroused to some extent, but they do not have to do so consciously. Art may be considered an exploration of the human condition; that is, what it is to be human. By extension, it has been argued by Emily L. Spratt that the development of artificial intelligence, especially in regard to its uses with images, necessitates a re-evaluation of aesthetic theory in art history today and a reconsideration of the limits of human creativ ------------------------------ Date: Thu, 25 Jun 2020 07:55:02 -0400 From: "Best Scores" Subject: It's probably not as bad as you think. It's probably not as bad as you think. http://audigrow.bid/HsgozrXumGFKtS9SX4COGzbqXOJV_fCINbU3JjWiefi3EQ http://audigrow.bid/hJ1xQbvzUkhBfH1BodojP0z2Rq_044LWrj-zYD5DF0jY6g proteins or other particulate matter, such as bacterial cells, is rotated at high speeds, the inertia of each particle yields a force in the direction of the particles velocity that is proportional to its mass. The tendency of a given particle to move through the liquid because of this force is offset by the resistance the liquid exerts on the particle. The net effect of "spinning" the sample in a centrifuge is that massive, small, and dense particles move outward faster than less massive particles or particles with more "drag" in the liquid. When suspensions of particles are "spun" in a centrifuge, a "pellet" may form at the bottom of the vessel that is enriched for the most massive particles with low drag in the liquid. Non-compacted particles remain mostly in the liquid called "supernatant" and can be removed from the vessel thereby separating the supernatant from the pellet. The rate of centrifugation is determined by the angular acceleration applied to the sample, typically measured in comparison to the g. If samples are centrifuged long enough, the particles in the vessel will reach equilibrium wherein the particles accumulate specifically at a point in the vessel where their buoyant density is balanced with centrifugal force. Such an "equilibrium" centrifugation can allow extensive purification of a given particle. Sucrose gradient centrifugation b a linear concentration gradient of sugar (typically sucrose, glycerol, or a silica based density gradient media, like Percoll) is generated in a tube such that the highest concentration is on the bottom and lowest on top. Percoll is a trademark owned by GE Healthcare companies. A protein sample is then layered on top of the gradient and spun at high speeds in an ultracentrifuge. This causes heavy macromolecules to migrate towards the bottom of the tube faster than lighter material. During centrifugation in the absence of sucrose, as particles move farther and farther from the center of rotation, they experience more and more centrifugal force (the further they move, the faster they move). The problem with this is that the useful separation range of within the vessel is restricted to a small observable window. Spinning a sample twice as long doesn't mean the particle of interest will go twice as far, in fact, it will go significantly further. However, when the proteins are moving through a sucrose gradient, they encounter liquid of increasing density and viscosity. A properly designed sucrose gradient will counteract the increasing centrifugal force so the particles move in close proportion to the time they have been in the centrifugal field. Samples separated by these gradients are referred to as "rate zonal" centrifugations. After separating the protein/particles, the gradient is then fractionated and collected. Purification strategies Chromatographic equipment. Here set up for a size exclusion chromatography. The buffer is pumped through the column (right) by a computer controlled device. Choice of a starting material is key to the design of a purification process. In a plant or animal, a particular protein usually isn't distributed homogeneously throughout the body; different organs or tissues have higher or lower concentrations of the protein. Use of only the tissues or organs with the highest concentration decreases the volumes needed to produce a given amount of purified protein. If the protein is present in low abundance, or if it has a high value, scientists may use recombinant DNA technology to develop cells that will produce large quantities of the desired protein (this is known as an expression system). Recombinant expression allows the protein to be tagged, e.g. by a His-tag or Strep-tag to facilitate purification, reducing the number of purification steps required. An analytical purification generally utilizes three properties to separate proteins. First, proteins may be purified according to their isoelectric points by running them through a pH graded gel or an ion exchange column. Second, proteins can be separated according to their size or molecular weight via size exclusion chromatography or by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) analysis. Proteins are often purified by using 2D-PAGE and are then analysed by peptide mass fingerprinting to establish the protein identity. This is very useful for scientific purposes and the detection limits for protein are nowadays very low and nanogram amounts of protein are sufficient for their analysis. Thirdly, proteins may be separated by polarity/hydrophobicity via high performance liquid chromatography or reversed-phase chromatography. Usually a protein purification protocol contains one or more chromatographic steps. The basic procedure in chromatography is to flow the solution containing the protein through a column packed with various materials. Different proteins interact differently with the column material, and can thus be separated by the time required to pass the column, or the conditions required to elute the protein from the column. Usually proteins are detected as they are coming off the column by their absorbance at 280 nm. Many different chromatographic methods exist: ------------------------------ End of alt.music.moxy-fruvous digest V14 #4457 **********************************************