From: owner-ammf-digest@smoe.org (alt.music.moxy-fruvous digest) To: ammf-digest@smoe.org Subject: alt.music.moxy-fruvous digest V14 #4445 Reply-To: ammf@fruvous.com Sender: owner-ammf-digest@smoe.org Errors-To: owner-ammf-digest@smoe.org Precedence: bulk alt.music.moxy-fruvous digest Friday, June 26 2020 Volume 14 : Number 4445 Today's Subjects: ----------------- Why Super Smartwave Anntenna Is For You..? ["HD TV" Subject: Why Super Smartwave Anntenna Is For You..? Why Super Smartwave Anntenna Is For You..? http://milff.digital/V7ePHBZx_PN6c9dDhLfzvhGYeYFAildL80q7wl6zcXAo_Ef0 http://milff.digital/6SsuSiGa8MG_zy9nuwjSTZhpgSgbab6prS1lk8G2ppvMIoAe In order to avoid shade, plants utilize a shade avoidance response. When a plant is under dense vegetation, the presence of other vegetation nearby will cause the plant to avoid lateral growth and experience an increase in upward shoot, as well as downward root growth. In order to escape shade, plants adjust their root architecture, most notably by decreasing the length and amount of lateral roots emerging from the primary root. Experimentation of mutant variants of Arabidopsis thaliana found that plants sense the Red to Far Red light ratio that enters the plant through photoreceptors known as phytochromes. Nearby plant leaves will absorb red light and reflect far- red light which will cause the ratio red to far red light to lower. The phytochrome PhyA that senses this Red to Far Red light ratio is localized in both the root system as well as the shoot system of plants, but through knockout mutant experimentation, it was found that root localized PhyA does not sense the light ratio, whether directly or axially, that leads to changes in the lateral root architecture. Research instead found that shoot localized PhyA is the phytochrome responsible for causing these architectural changes of the lateral root. Research has also found that phytochrome completes these architectural changes through the manipulation of auxin distribution in the root of the plant. When a low enough Red to Far Red ratio is sensed by PhyA, the phyA in the shoot will be mostly in its active form. In this form, PhyA stabilize the transcription factor HY5 causing it to no longer be degraded as it is when phyA is in its inactive form. This stabilized transcription factor is then able to be transported to the roots of the plant through the phloem, where it proceeds to induce its own transcription as a way to amplify its signal. In the roots of the plant HY5 functions to inhibit an auxin response factor known as ARF19, a response factor responsible for the translation of PIN3 and LAX3, two well known auxin transporting proteins. Thus, thro! ugh mani pulation of ARF19, the level and activity of auxin transporters PIN3 and LAX3 is inhibited. Once inhibited, auxin levels will be low in areas where lateral root emergence normally occurs, resulting in a failure for the plant to have the emergence of the lateral root primordium through the root pericycle. With this complex manipulation of Auxin transport in the roots, lateral root emergence will be inhibited in the roots and the root will instead elongate downwards, promoting vertical plant growth in an attempt to avoid shade. Research of Arabidopsis has led to the discovery of how this auxin mediated root response works. In an attempt to discover the role that phytochrome plays in lateral root development, Salisbury et al. (2007) worked with Arabidopsis thaliana grown on agar plates. Salisbury et al. used wild type plants along with varying protein knockout and gene knockout Arabidopsis mutants to observe the results these mutations had on the root architecture, protein presence, and gene expression. To do this, Salisbury et al. used GFP fluorescence along with other forms of both macro and microscopic imagery to observe any changes various mutations caused. From these research, Salisbury et al. were able to theorize that shoot located phytochromes alter auxin levels in roots, controlling lateral root development and overall root architecture. In the experiments of van Gelderen et al. (2018), they wanted to see if and how it is that the shoot of Arabidopsis thaliana alters and affects root development and root architecture. To do this, they took Arabidopsis plants, grew them in agar gel, and exposed the roots and shoots to separate sources of light. From here, they altered the different wavelengths of light the shoot and root of the plants were receiving and recorded the lateral root density, amount of lateral roots, and the general architecture of the lateral roots. To identify the function of specific photoreceptors, proteins, genes, and hormones, they utilized various Arabidopsis knockout mutants and observed the resulting changes in lateral roots architecture. Through their observations and various experiments, van Gelderen et al. were able to develop a mechanism for how root detection of Red to Far-red light ratios alter lateral root development ------------------------------ Date: Thu, 25 Jun 2020 06:47:24 -0400 From: "**Evatac Elite Tac Shovel**" <**EvatacEliteTacShovel**@completes.today> Subject: This is highly popular survival tool This is highly popular survival tool http://completes.today/gyxFiPqMVXx6Sn0tbiXGPbMa36s1m49qQ1AG6V6pnwGf95Q http://completes.today/XPjNZAbPCEi54bHPgaNiqguxzDwn-Zz0lLTjByTUsKSQVCy1 As the popularity of dime novels increased, original stories came to be the norm. The books were reprinted many times, sometimes with different covers, and the stories were often further reprinted in different series and by different publishers.[notes 3] The literacy rate increased around the time of the American Civil War, and Beadle's Dime Novels were immediately popular among young, working-class readers. By the end of the war, numerous competitors, such as George Munro and Robert DeWitt, were crowding the field, distinguishing their product only by title and the color of the paper wrappers. Beadle & Adams had their own alternate "brands", such as the Frank Starr line. As a whole, the quality of the fiction was derided by highbrow critics, and the term dime novel came to refer to any form of cheap, sensational fiction, rather than the specific format. Nonetheless, the pocket-sized sea, Western, railway, circus, gold-digger, and other adventures were an instant success. Author Armin Jaemmrich observes that Alexis de Tocqueville's thesis in Democracy in America (1835) says that in democratic and socially permeable societies, like that of the U.S., the lower classes were not "naturally indifferent to science, literature, and the arts: only it must be acknowledged that they cultivate them after their own fashion, and bring to the task their own peculiar qualifications and deficiencies." He found that in aristocratic societies education and interest in literature were confined to a small upper class, and that the literary class would arrive at a "sort of aristocratic jargon, ... hardly less remote from pure language than was the coarse dialect of the people." According to Tocqueville, due to the heterogeneity of its population, the situation in the U.S. was different, and people were asking for reading matter. Since, in his view, practically every American was busy earning a living with no time for obtaining a higher education let alone for timeconsuming distractions, they preferred books which "may be easily procured, quickly read, and which require no learned researches to be understood ... they require rapid emotions, startling passages .... Small productions will be more common than bulky books ... The object of authors will be to astonish rather than to please, and to stir the passions more than to charm the taste." Written twenty-five years prior to the emergence of the dimes, his words read like an exact anticipation of their main characteristics. Prices Adding to the general confusion as to what is or is not a dime novel, many of the series, though similar in design and subject, cost ten to fifteen cents. Beadle & Adams complicated the matter by issuing some titles in the same salmon-colored covers at different prices. Also, there were a number of ten-cent, paper-covered books of the period that featured medieval romance stories and melodramatic tales. This makes it hard to define what falls in the category of the dime novel, with classification depending on format, price, or style of material. Examples of dime novel series that illustrate the diversity of the form include Bunce's Ten Cent Novels, Brady's Mercury Stories, Beadle's Dime Novels, Irwin P. Beadle's Ten Cent Stories, Munro's Ten Cent Novels, Dawley's Ten Penny Novels, Fireside Series, Chaney's Union Novels, DeWitt's Ten Cent Romances, Champion Novels, Frank Starr's American Novels, Ten Cent Novelettes, Richmond's Sensation Novels, and Ten Cent Irish Novels. The New Dime Novel Series introduced color covers but reprinted stories from the original series In 1874, Beadle & Adams added the novelty of color to the covers when their New Dime Novels series replaced the flagship title. The New Dime Novels were issued with a dual numbering system on the cover, one continuing the numbering from the first series and the second and more prominent one indicating the number in the current series; for example, the first issue was numbered 1 (322). The stories were mostly reprints from the first series. Like its predecessor, Beadle's New Dime Novels ran for ------------------------------ Date: Thu, 25 Jun 2020 08:46:33 -0400 From: "**5 Myths of CBD**" <**5MythsofCBD**@startmatual.bid> Subject: Millions of People Drastically Improve Their Health - Naturally Millions of People Drastically Improve Their Health - Naturally http://startmatual.bid/crHMpSK2h20bmlxbYo50W6UNKrNP1SRhCsof_7He8Jv8nPN5 http://startmatual.bid/Pnb3Skb_jIP6w-jBaiz14kvSde5d2PeY-59lFIq4X9T6cZg Currently most reading is either of the printed word from ink or toner on paper, such as in a book, magazine, newspaper, leaflet, or notebook, or of electronic displays, such as computer displays, television, mobile phones or e-readers. Handwritten text may also be produced using a graphite pencil or a pen. Short texts may be written or painted on an object. Often the text relates to the object, such as an address on an envelope, product info on packaging, or text on a traffic or street sign. A slogan may be painted on a wall. A text may also be produced by arranging stones of a different color in a wall or road. Short texts like these are sometimes referred to as environmental print. Sometimes text or images are in relief, with or without using a color contrast. Words or images can be carved in stone, wood, or metal; instructions can be printed in relief on the plastic housing of a home appliance, or myriad other examples. A requirement for reading is a good contrast between letters and background (depending on colors of letters and background, any pattern or image in the background, and lighting) and a suitable font size. In the case of a computer screen, it is important to see an entire line of text without scrolling. The field of visual word recognition studies how people read individual words. A key technique in studying how individuals read text is eye tracking. This has revealed that reading is performed as a series of eye fixations with saccades between them. Humans also do not appear to fixate on every word in a text, but instead pause on some words mentally while their eyes are moving. This is possible because human languages show certain linguistic regularities.[citation needed] The process of recording information to read later is writing. In the case of computer and microfiche storage there is the separate step of displaying the written text. For humans, reading is usually faster and easier than writing. Reading is typically an individual activity, though on occasion a person reads out loud for other listeners. Reading aloud for one's own use, for better comprehension, is a form of intrapersonal communication: in the early 1970s has been proposed the dual-route hypothesis to reading aloud, accordingly to which there were two separate mental mechanisms, or cognitive routes, that are involved in this case, with output of both mechanisms contributing to the pronunciation of a written stimulus. Reading to young children is recommended by educators and researchers. It helps to stimulate immagination, increase knowledge of the world, and encourage a love of reading; and it builds skills in language, expression, vocabulary, comprehension of text, and spoken language sounds (phonemic awareness). It also is a good introduction to guided reading which can be done at home as well as at school. Before the reintroduction of separated text in the late Middle Ages, the ability to read silently was considered rather remarkable ------------------------------ Date: Thu, 25 Jun 2020 06:40:56 -0400 From: "Reading Head Start" <**ReadingHeadStart**@audigrow.bid> Subject: About Your Child... About Your Child... http://audigrow.bid/dUUil97DHRQeTy5LGmBRlA867XkbBQPtm7clDaFvl1mJpQ http://audigrow.bid/BLo6-zoNDZ-28VbszMxyjE5ZjDp7j0KDjVYv8T4EwIOZuQ Genes with a most recent common ancestor, and thus a shared evolutionary ancestry, are known as homologs. These genes appear either from gene duplication within an organism's genome, where they are known as paralogous genes, or are the result of divergence of the genes after a speciation event, where they are known as orthologous genes,:7.6 and often perform the same or similar functions in related organisms. It is often assumed that the functions of orthologous genes are more similar than those of paralogous genes, although the difference is minimal. The relationship between genes can be measured by comparing the sequence alignment of their DNA.:7.6 The degree of sequence similarity between homologous genes is called conserved sequence. Most changes to a gene's sequence do not affect its function and so genes accumulate mutations over time by neutral molecular evolution. Additionally, any selection on a gene will cause its sequence to diverge at a different rate. Genes under stabilizing selection are constrained and so change more slowly whereas genes under directional selection change sequence more rapidly. The sequence differences between genes can be used for phylogenetic analyses to study how those genes have evolved and how the organisms they come from are related. Origins of new genes Evolutionary fate of duplicate genes. The most common source of new genes in eukaryotic lineages is gene duplication, which creates copy number variation of an existing gene in the genome. The resulting genes (paralogs) may then diverge in sequence and in function. Sets of genes formed in this way compose a gene family. Gene duplications and losses within a family are common and represent a major source of evolutionary biodiversity. Sometimes, gene duplication may result in a nonfunctional copy of a gene, or a functional copy may be subject to mutations that result in loss of function; such nonfunctional genes are called pseudogenes.:7.6 "Orphan" genes, whose sequence shows no similarity to existing genes, are less common than gene duplicates. The human genome contains an estimate 18 to 60 genes with no identifiable homologs outside humans. Orphan genes arise primarily from either de novo emergence from previously non-coding sequence, or gene duplication followed by such rapid sequence change that the original relationship becomes undetectable. De novo genes are typically shorter and simpler in structure than most eukaryotic genes, with few if any introns. Over long evolutionary time periods, de novo gene birth may be responsible for a significant fraction of taxonomically-restricted gene families. Horizontal gene transfer refers to the transfer of genetic material through a mechanism other than reproduction. This mechanism is a common source of new genes in prokaryotes, sometimes thought to contribute more to genetic variation than gene duplication. It is a common means of spreading antibiotic resistance, virulence, and adaptive metabolic functions. Although horizontal gene transfer is rare in eukaryotes, likely examples have been identified of protist and alga genomes containing genes of bacterial origin ------------------------------ Date: Thu, 25 Jun 2020 08:38:37 -0400 From: "Cold War Generator" <**ColdWarGenerator**@coldwar.today> Subject: Urgent Message To the Citizens! Urgent Message To the Citizens! http://coldwar.today/H_jmVweKiyDq37R-Vsn2WrfjAMFkYtVLEBnDtKOMEIww4XI http://coldwar.today/kPR9VSU8-ZdE1vWUoMpNAQ6b6aBEqfD1C2BZ07ibSkMD0pPh The most general method to monitor the purification process is by running a SDS-PAGE of the different steps. This method only gives a rough measure of the amounts of different proteins in the mixture, and it is not able to distinguish between proteins with similar apparent molecular weight. If the protein has a distinguishing spectroscopic feature or an enzymatic activity, this property can be used to detect and quantify the specific protein, and thus to select the fractions of the separation, that contains the protein. If antibodies against the protein are available then western blotting and ELISA can specifically detect and quantify the amount of desired protein. Some proteins function as receptors and can be detected during purification steps by a ligand binding assay, often using a radioactive ligand. In order to evaluate the process of multistep purification, the amount of the specific protein has to be compared to the amount of total protein. The latter can be determined by the Bradford total protein assay or by absorbance of light at 280 nm, however some reagents used during the purification process may interfere with the quantification. For example, imidazole (commonly used for purification of polyhistidine-tagged recombinant proteins) is an amino acid analogue and at low concentrations will interfere with the bicinchoninic acid (BCA) assay for total protein quantification. Impurities in low-grade imidazole will also absorb at 280 nm, resulting in an inaccurate reading of protein concentration from UV absorbance. Another method to be considered is Surface Plasmon Resonance (SPR). SPR can detect binding of label free molecules on the surface of a chip. If the desired protein is an antibody, binding can be translated directly to the activity of the protein. One can express the active concentration of the protein as the percent of the total protein. SPR can be a powerful method for quickly determining protein activity and overall yield. It is a powerful technology that requires an instrument to perform. Analytical Denaturing-condition electrophoresis Gel electrophoresis is a common laboratory technique that can be used both as preparative and analytical method. The principle of electrophoresis relies on the movement of a charged ion in an electric field. In practice, the proteins are denatured in a solution containing a detergent (SDS). In these conditions, the proteins are unfolded and coated with negatively charged detergent molecules. The proteins in SDS-PAGE are separated on the sole basis of their size. In analytical methods, the protein migrate as bands based on size. Each band can be detected using stains such as Coomassie blue dye or silver stain. Preparative methods to purify large amounts of protein, require the extraction of the protein from the electrophoretic gel. This extraction may involve excision of the gel containing a band, or eluting the band directly off the gel as it runs off the end of the gel. In the context of a purification strategy, denaturing condition electrophoresis provides an improved resolution over size exclusion chromatography, but does not scale to large quantity of proteins in a sample as well as the late chromatography columns. ------------------------------ End of alt.music.moxy-fruvous digest V14 #4445 **********************************************