From: owner-ammf-digest@smoe.org (alt.music.moxy-fruvous digest) To: ammf-digest@smoe.org Subject: alt.music.moxy-fruvous digest V14 #11615 Reply-To: ammf@fruvous.com Sender: owner-ammf-digest@smoe.org Errors-To: owner-ammf-digest@smoe.org Precedence: bulk alt.music.moxy-fruvous digest Tuesday, June 13 2023 Volume 14 : Number 11615 Today's Subjects: ----------------- mouth bacteria ["Lana" ] ---------------------------------------------------------------------- Date: Tue, 13 Jun 2023 15:29:05 +0200 From: "Lana" Subject: mouth bacteria mouth bacteria http://samsurveymachine.shop/OlAYQ-13rLkFp2wKvg65lWz3FGh_DAdWhdPX75PL0Q8-1GYfKg http://samsurveymachine.shop/lysDCx4Tl4CGA2jcrYjF7iKFyoTorR7J0WMoVa-5tIuOS4ImPA Analyzing proteins proves to be more difficult than analyzing nucleic acid sequences. While there are only 4 nucleotides that make up DNA, there are at least 20 different amino acids that can make up a protein. Additionally, there is currently no known high throughput technology to make copies of a single protein. Numerous methods are available to study proteins, sets of proteins, or the whole proteome. In fact, proteins are often studied indirectly, e.g. using computational methods and analyses of genomes. Only a few examples are given below. Separation techniques and electrophoresis Proteomics, the study of the proteome, has largely been practiced through the separation of proteins by two dimensional gel electrophoresis. In the first dimension, the proteins are separated by isoelectric focusing, which resolves proteins on the basis of charge. In the second dimension, proteins are separated by molecular weight using SDS-PAGE. The gel is stained with Coomassie brilliant blue or silver to visualize the proteins. Spots on the gel are proteins that have migrated to specific locations. Mass spectrometry An Orbitrap mass spectrometer commonly used in proteomics Main articles: Protein mass spectrometry and Mass spectrometry Mass spectrometry is one of the key methods to study the proteome. Some important mass spectrometry methods include Orbitrap Mass Spectrometry, MALDI (Matrix Assisted Laser Desorption/Ionization), and ESI (Electrospray Ionization). Peptide mass fingerprinting identifies a protein by cleaving it into short peptides and then deduces the protein's identity by matchin ------------------------------ End of alt.music.moxy-fruvous digest V14 #11615 ***********************************************